Broad Phenotypic Classes
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Abnormal Chromosome Structure or Nuclear Morphology
(24 genes in 16 phenotypic classes)
Abnormal nuclear morphology (misshapen, large, small, or fragmented nuclei) and/or abnormal chromatin structure (decondensed or hypercondensed) is observed.
Abnormal Proliferation and Cell Cycle Progression
(5 genes in 2 phenotypic classes)
Gonad contains either too many or too few nuclei, indicative of over or under-proliferation. Nuclei are also frequently in an abnormal cell cycle state.
Abrupt Compartment Expansion
(8 genes in 3 phenotypic classes)
In contrast to the gradual increase in compartment size in controls, expansion is abrupt, with large compartments immediately adjacent to small compartments.
Absent Ring Canals, Increased Clustered Apoptosis & Delayed Oocyte Budding
(1 genes in 1 phenotypic class)
Oocytes bud off the rachis later than in controls (typically at the -4 oocyte). The ring canals connecting individual compartments to the rachis appear sealed/missing. Apoptotic corpses are abundant and clustered.
Apoptosis Absent
(1 genes in 1 phenotypic class)
No apoptotic corpses visable. Control gonads have 3-5 visable corpses in the images region.
Decondensed Chromatin and Irregular Oocyte Junctions
(20 genes in 2 phenotypic classes)
Oocytes have jagged irregular boundaries, decondensed chromatin, and internal PI4,5P2. Compartments begin to expand in the distal region of the gonad rather than at the turn.
Decreased Oocyte Number
(9 genes in 2 phenotypic classes)
Gonad has less than 4 oocytes that are budded off from the rachis.
Degenerate Gonad
(7 genes in 1 phenotypic class)
The presence of old embryos and/or hatched larvae, but no young embryos, in the uterus indicates that the gonad initially produced viable embryos but subsequently ceased to function. Typically accompanied by a narrow distal region/rachis, abrupt expansion, and severely reduced oocyte number.
Delayed Oocyte Budding
(74 genes in 8 phenotypic classes)
Oocytes fail to bud off the rachis in a timely manner.
Delayed or Incomplete Compartment expansion
(110 genes in 12 phenotypic classes)
Syncytial compartments begin to expand proximal to the turn, rather than at the turn.
Distal Constriction or Pathfinding
(4 genes in 2 phenotypic classes)
Distal region of gonad contains a contriction and/or the distal tip is abnormally positioned with respect to the turn.
Distorted Proximal Oocyte & Embryos That Fail Cell Division
(5 genes in 1 phenotypic class)
Proximal oocyte is severely deformed, gonad is otherwise wild-type. Single cell embryos containing multiple nuclei accumulate in the uterus.
Enlarged Vesicles at Rachis Tip & PI4,5P2 Debris
(4 genes in 1 phenotypic class)
Proximal end of rachis terminates in a series of large vesicles. Compartment sizes are irregular and the cytoplasm contains PI4,5P2.
Extraneous Germline Material
(2 genes in 1 phenotypic class)
Germline material appears outside of the gonad proper.
Increased Oocyte Number
(2 genes in 2 phenotypic classes)
More than 4 budded oocytes are present in the gonad.
Internal Comparments in Rachis
(15 genes in 2 phenotypic classes)
Compartments span the width of the rachis. In controls, the compartments are limited to the periphery of the gonad.
Large Nuclei in Proximal Oocyte & Disintegrating Oocyte Partitions
(1 genes in 1 phenotypic class)
The nuclei in proximal oocytes are larger than in control oocytes, and the oocyte boundaries are disintegrating or absent.
Mosaic Proximal Region and Severe Punctate PI4,5P2
(3 genes in 1 phenotypic class)
"Oocytes are irregularly shaped and fit together in a ""mosaic"" pattern. Cytoplasm is filled with punctate PI4,5P2. Progression through diplotene and/or diakinesis is abnormal."
Multinucleation
(44 genes in 13 phenotypic classes)
More than one nucleus is present in a single compartment or oocyte
Multinucleation & Incomplete Partitions
(18 genes in 4 phenotypic classes)
More than one nucleus is present in a single compartment or oocyte, and some or all partitions between compartments are incomplete or absent
Nuclear Fallout or Near Fallout
(39 genes in 5 phenotypic classes)
Nuclei are mispositioned within compartments and/or have fallen out of the compartments into the rachis
Partitions Incomplete with Peripheral Nuclei and PI4,5P2 Debris
(5 genes in 1 phenotypic class)
The partitions that separate compartments are absent or partially absent throughout gonad and internal PI4,5P2-labeled debris fills the cytoplasm. The rachis is wide and the nuclei remain along the periphery of the gonad.
Premature Compartment Expansion
(37 genes in 4 phenotypic classes)
Syncytial compartments begin to expand distal to the turn, rather than at the turn.
Rounded Compartments
(7 genes in 2 phenotypic classes)
Syncytial compartments are rounded (rather than squared off) and are nearly equal in size to small rounded oocytes in the proximal region
Small Bright PI4,5P2 Foci & Decreased Oocyte Number
(2 genes in 1 phenotypic class)
Gonad has small bright foci of PI4,5P2 in the cytoplasm and has fewer oocytes than controls.
Small or L4-like Gonad
(46 genes in 4 phenotypic classes)
Gonad appears similar to that normally seen at earlier developmental stages. Compartments fail to expand and rachis is absent or extremely narrow.
Vesiculated Oocyte Junctions, Convoluted Rachis & Annucleate Compartments
(8 genes in 1 phenotypic class)
The boundaries between oocytes often consist of small vesicles and anucleate compartments are frequently present. The rachis is convoluted and the diplotene region is shorter than in controls.
Vesiculation Throughout
(25 genes in 6 phenotypic classes)
Plasma membrane derived vesicles fill the rachis and surround the compartments.
Wild-Type Gonad Morphology
(33 genes in 2 phenotypic classes)
Mostly wild-type gonad morphology- may have one or two minor scored defects.